Journal: International Journal of Molecular Sciences

Article Title: High Adenosine Extracellular Levels Induce Glioblastoma Aggressive Traits Modulating the Mesenchymal Stromal Cell Secretome

doi: 10.3390/ijms21207706

Figure Lengend Snippet: Effects of ADO in the BM-MSC and GBM cell cross-talk. ( A ) The image depicts the contact-independent transwell cultures with BM-MSCs in the upper chamber and U343MG in the lower chamber. U343MG cell proliferation was evaluated after 24 and 48 h with Crystal violet, as reported in . ( B ) The image depicts the contact-independent transwell cultures with U343MG in the upper chamber and BM-MSCs in the lower chamber. BM-MSCs cell proliferation was evaluated after 48 h with Crystal violet, as reported in . ( C ) U343MG cells were seeded in the upper chamber and BM-MSCs treated with ADO (100 nM and 100 µM) in the lower chamber. After 24 h, U343MG cell invasion was analyzed using Matrigel basement membrane transwell system, as described in . Representative images are shown. Cell migration was quantified by counting the number of cells that migrated into the lower surface of the transwell membrane. The data are reported as percentage of cell invasion versus the CTRL set to 100% and are the means ± SEM of three independent experiments. The significance of the differences was determined by one-way ANOVA, followed by Bonferroni’s post-hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CTRL.

Article Snippet: Human bone marrow MSCs (BM-MSCs) and glioblastoma stem cells (U343MG) were purchased by CLS (CLS Cell Lines Service GmbH, Eppelheim, Germany).

Techniques: Migration

Journal: PLOS ONE

Article Title: Recovery after human bone marrow mesenchymal stem cells (hBM-MSCs)-derived extracellular vesicles (EVs) treatment in post-MCAO rats requires repeated handling

doi: 10.1371/journal.pone.0312298

Figure Lengend Snippet: (a–c) Characterization and quantification of human-bone marrow mesenchymal stem cell (hBM-MSC)-derived extracellular vesicles (EVs). (a) Nanosight Tracking analyses (NTA) showing the size distribution pattern of EVs. (b) Western blots of hBM-MSCs marker (GM130) and EVs markers (ALIX, CD9, CD81). “Wash” lane represents the negative control. Each image comes from different blots. (c) Representative image of EVs using transmission electron microscopy (TEM). Scale bar 100 nm. (d–f) In vivo tracking of ExoGlow-labeled EVs demonstrated that EVs reach the brain and accumulate near the stroke injury 6 hours after administration at 2 dps. (d) Timeline of intranasal single dose treatment of ExoGlow-labeled EVs (~2.4 x 10 9 EVs in 200 μl), starting 2 dps and collecting the tissue 6 hours after. (e) Representative coronal section after a single intranasal administration of ExoGlow-labeled EVs (~2.4 x 10 9 EVs in 200 μl) at 2 dps. Hoechst indicates cell nuclei. Scale bars 4 mm and 100 μm. (f) Quantification of the average size of ExoGlow+ particles in the ipsilateral and contralateral hemispheres. Data was analyzed using a paired t-test (*p<0.05). Bars show mean +/− SD. Each symbol represents the average size of ExoGlow+ particles from all ROIs in each hemisphere (5 ROIs/hemisphere in each rat) of a single rat ( n = 4 ).

Article Snippet: Human bone marrow mesenchymal stem cells (hBM-MSCs) were purchased from Celprogren (frozen vial with ~1.2 x 10 6 cells; #36094–22) and plated on human mesenchymal bone marrow stem cell culture extracellular expansion matrix pre-coated T75 Flasks (Celprogen, #E36094-21-T75) following Celprogren recommendations.

Techniques: Derivative Assay, Western Blot, Marker, Negative Control, Transmission Assay, Electron Microscopy, In Vivo, Labeling

Journal: Scientific Reports

Article Title: GBR membrane of novel poly (butylene succinate-co-glycolate) co-polyester co-polymer for periodontal application

doi: 10.1038/s41598-018-25952-1

Figure Lengend Snippet: Morphology of rabbit MSCs on PBSGL nanofibers (PBSGL0 ( a ), PBSGL10 ( b ), BSGL20 ( c ) and PBSGL40 ( d )) seeded at a cell density of 4000 cells per cm 2 after 7 days’ culture. Cells were immuno-stained with an antibody against vinculin (green), FITC–phalloidin (red), and DAPI (blue). Scale bar = 100 μm.

Article Snippet: Bone marrow-derived MSCs from the femur of a 3.5 kg, male New Zealand white rabbit at skeletal maturity were ordered from the National Cell Bank, Pasteur Institute of Iran.

Techniques: Staining

Journal: Scientific Reports

Article Title: GBR membrane of novel poly (butylene succinate-co-glycolate) co-polyester co-polymer for periodontal application

doi: 10.1038/s41598-018-25952-1

Figure Lengend Snippet: Metabolic activity (proliferation) of rabbit MSCs cultured on electrospun nanofibers submerged in basal medium at day 1, 3, 7, and 12. The secondary y axis shows the number of rabbit MSCs for colored dashed lines corresponding to each group. An asterisk denotes a statistically significant difference (P < 0.05) between the test and control group at each time point. The sample size selected was 5.

Article Snippet: Bone marrow-derived MSCs from the femur of a 3.5 kg, male New Zealand white rabbit at skeletal maturity were ordered from the National Cell Bank, Pasteur Institute of Iran.

Techniques: Activity Assay, Cell Culture, Control

Journal: Scientific Reports

Article Title: GBR membrane of novel poly (butylene succinate-co-glycolate) co-polyester co-polymer for periodontal application

doi: 10.1038/s41598-018-25952-1

Figure Lengend Snippet: Osteogenic differentiation of rabbit MSCs cultured on electrospun PBSGL n membranes at days 4, 7, 14, and 21 in the osteogenic media: DNA ( a ); ALP activity ( b ); calcium content ( c ); gene expression (Col-α1 ( d ), Runx-2 ( e ), and OCN ( f )); protein expressions of OCN and Col-α1 at day 21 relative to β actin expression ( g ); Western blotting bands for OCN (12 kDa), Col-α1 (130 kDa), and β actin (42 kDa) (h). Three different gels (with the same acquisition settings and exposure parameters) were used to visualize the relative protein expressions of OCN, Col-α1 and β actin proteins. Alizarin Red staining showing deposited calcium ions on the electrospun PBSGL0 (i-1), PBSGL10, (i-2) PBSGL20, (i-3) and PBSGL40 (i-4) nanofibers at day 21. An asterisk denotes a statistically significant difference P < 0.05) between the test and control group at each time point. A “#” sign indicates a statistically significant difference between the test and all other groups at each time point. Sample size selected was 5.

Article Snippet: Bone marrow-derived MSCs from the femur of a 3.5 kg, male New Zealand white rabbit at skeletal maturity were ordered from the National Cell Bank, Pasteur Institute of Iran.

Techniques: Cell Culture, Activity Assay, Gene Expression, Expressing, Western Blot, Staining, Control